Genetic Fingerprinting of Methicillin Resistant Staphlococcus anreus Strains in Patients With Urinary Tract Infections (UTIs)

Resistant to methicillin in Staphylococcus aureus is responsible for human, community and live-stock infections in the world and has become a worldwide challenge of great concern. The aim of this study was to analyze the molecular diversity among methicillin resistant Staphylococcus aureus (MRSA) strains obtained from patients with urinary tract infection from hospitals in Lagos State. Out of eighty isolates of Staphylococcus aureus used in this study, 28 (35%) were confirmed methicillin resistant Staphylococcus aureus (MRSA) while 52(65%) were methicillin susceptible Staphylococcus aureus (MSSA) with disk diffusion method of Kirby-Bauer. Random amplified polymorphic DNA (RAPD)-PCR was applied on 22 isolates with high level of methicillin resistance using three oligonucleotides random primers (OPT -02). OPT-08 and OPT-17 except an isolate showing no banding patterns. However, polymorphisms were observed in all isolates using Primers OPT-08 and OPT-17 excepts an isolate showing no amplification with primer OPT-17. The two primers that yielded discriminatory patterns were used to perform the RAPD analysis. Products of the PCR generated many polymorphic bands between the range of >100 to 1200 base pairs in size. Highest number of bands was produced by primer OPT -08 with different distinct polymorphism.