In Vitro Propagation of Olive Plant (olea europea) "SOURT" Using Nodal Culture

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dc.contributor.advisorDr. K. E. Ogunsola
dc.contributor.authorKALEJAIYE, Olusegun Olanrewaju
dc.date.accessioned2026-08-27T11:21:05Z
dc.date.available2026-08-27T11:21:05Z
dc.date.issued2016-08-19
dc.descriptionxii,50pages,illustration;.hardback
dc.description.abstractThis study was undertaken to contribute to the previous knowledge of the efficient in vitro protocols for the micropropagation of olive plant. This work was based on development of a new in vitro propagation method to overcome growth recalcitrance an olive cultivar "souri". An experiment to determine optimum sterilization procedures for olive explants was conducted. The culture medium used was composed of Murashige and Skoog (MS) medium without hormonal modification and MS medium modified with various levels of auxin-cytokinin concentrations. The experiments were laid out in completely randomized design (CRD) with 10 replicates per media treatment. Data were collected at 2,4 and 6 weeks of culture on growth parameters which include frequency of buds formed, frequency of callus formation, number of leaves, leave length, leaf width, number of roots and root length. Data were analyzed by ANOVA using SAS programme (9.2 version) and means were separated by the Duncan multiple range test at 5% probability. Of the different sterilization treatments used on the explants during the establishment of culture, a treatment of 70% ethanol for 3minutes and 5% Sodium hypochlorite (NaOCI) for 2minutes respectively proved to be the best sterilant combination for the Olive explants. This treatment resulted in 91% survival of explants and zero death of explant after 21 days of culture. The Olive plant had the best initiation and proliferation in culture medium containing 2.0 mg/l BAP (treatment 3) followed by the medium modified with 0.05 mg/l BAP + 0.02 NAA. The parameters considered as evidence of success was bud formation, callus formation, number of leaves, length of leaves and width of leaves. The mean number of shoots produced in the medium supplemented with 2.0 mg/l BAP was (2.3 # 0.2) while the mean number of shoots obtained from Olive nodal cutting in MS medium containing the hormonal modification with the second best effect was (1.6 # 0.1). This hormonal treatment with the best mean number of shoot production (2.0 BAP mg/l), had the highest mean shoot length of (6.5 ‡ 0.3 ) and a mean leave width of (3.1 # 0.2 ). Murashige and Skoog medium supplemented with 0.05 mg/l IBA + 0.02mg/l NAA (treatment 4), gave the Olive plant the best rooting effects. The highest root number was 3.8 # 0.9 and that of the root length was 2.5 # 0.7. This study provides a media protocol for the in vitro regeneration of the Souri Cultivar of Olive which will foster the application of tissue culture techniques in the micropropagation of Olive plant and also enhance improvement of the plant through transformation.
dc.identifier.urihttps://ir.bellsuniversity.edu.ng/handle/123456789/516
dc.language.isoen
dc.publisherBells University of Technology
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectformation
dc.subjectprotocols
dc.titleIn Vitro Propagation of Olive Plant (olea europea) "SOURT" Using Nodal Culture
dc.typeDissertation

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