Browsing by Author "USMAN, Rahila Ukwo"

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    Effect of Spent and Non-Spent Engine Oil Contaminanted Soil on th DNA Profile of Oyster Mushroom
    (Bells University of Technology, 2016-09-20) USMAN, Rahila Ukwo; Dr. (Mrs) C. I. Anyakorah
    The effect of spent and non-spent engine oil on the morphology and genetic profile of P. tuber-regium (F.) singer was assessed. Five treatments each for the spent (S) engine oil (2%, 4%, 6%, 8% and 10%) and non-spent (NS) engine oil (2%, 4%, 6%, 8% and 10%) were applied to 1kg of soil in perforated polythene bags with P. tuber-regiumsclerotium planted in them. Percentage total organic matter, percentage nitrogen, phosphorus and pH values of the soil were 0.21%, 0.057%, 5% and 5.3 respectively. Soil analysis showed that S and NS had no effect on both the pH. The total organic matter and phosphorus contents of the spent treatments (S) and non-spent engine oil treatments (NS) increased when compared to the control. Total organic matter of the S and NS treated soils ranged from 0.22%-0.91% and 0.22-0.61 while the Phosphorus values for S and NS treated soils ranged from 4.5%-8.5% and 3.4%-9%. Nitrogen contents of the spent treatments (S) and non-spent engine oil treatments (NS) decreased when compared to the control ranging from 0.0378%-0.0325% (S) and 0.0308-0.01120 (NS). Diameter of cap (DOC), Width of stalk (WDS), weight of fruiting body (WOF) and length of stalk(LOS) of the soil were 6.75cm, 4.05cm, 5.75g and 3.5cm respectively. DOC ranged from 2.69cm-4.24cm(S) and 3.04cm-5.16cm(NS), WDS ranged from 3.87cm-4.11cm(S) and 5.63cm-8-12cm(NS), WOF ranged from 4.63g-6.69g(S) and 5.57g-14.03g(NS) while LOS ranged from 2.23cm-2.97cm(S) and 3.03-3.83(NS). No growth was obtained at 10% S and NS treatments. DNA was extracted from four mushroom samples(2S, 2NS, 4S, and 4NS) successfully. DNA quantification values ranged between 200.4ųg/ml and 695.1 ųg/ml.Amplification using ITS1 and ITS4B primers revealed a single monomorphic band at 600bp. The six RAPD primers produced total of 64 bands out of which 4.68% (3 bands) were monomorphic while 61 bands (95.31%) were polymorphic.