Browsing by Author "AKINTAN, Adekunle Azeez"

Now showing 1 - 1 of 1
  • No Thumbnail Available
    ItemMetadata only
    Molecule Detection of mecA and spa genes in Methicillin Resistant Staphylococcus aureus Bacteriuria
    (Bells University of Technology, 2021-05) AKINTAN, Adekunle Azeez; Dr. (Mrs) Seriki, A. T.
    Methicillin-resistant Staphylococcus aureus is a global public health concern that causes hospital and community-acquired infections all over the world. The emergence of MRSA is responsible for increasing health costs, patient morbidity and mortality. Asa result, a global priority list of antimicrobial-resistant bacteria designated it as Priority 2: HIGH. The aim of this study was to detect mecA and spA genes in methicillin -resistant Staphylococcus aureus bacteriuria obtained from patients with urinary tract infections. Eighty isolates were obtained from department of microbiology, Bells University of Technology, Ota, Ogun state. Out of which 74 were resuscitated. Antibiotic susceptibility test was carried out the 74 isolates for identification of methicillin resistant Staphylococcus aureus using cefoxitin (30 ug, Oxoid Ltd, Cambridge, UK) by Kirby Bauter disk diffusion method. Polymerase chain reaction was performed on twenty-two isolates showing high level phenotypic methicillin resistance for the presence of mecA and SpA genes. The result of the phenotypic methicillin resistance showed that 40 isolates out (40/74) were confirmed to be MRSA . However, only 8 isolates (36.4) out of 22 isolates were positive to meCA gene.The PCr result foe spa gene revealed only three (13.7%) positive isolates out of 22 (3/22). There was no statistically significant association between mecA and spA genes (p>0.05) in MRSA bacteriuria in UTI patients. This study showed high prevalence of MRSA using disk diffusion method but low prevalence of mecA gene among urinary tract infection patients in Lagos, NIgeria