Theses/Dissertations/Projects

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Browsing Theses/Dissertations/Projects by Author "Dr. O. B. Ojuederie"

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    Assessment of Genetic Diversity in Winged Bean (psophcarpus tetragonolobus) Using Inter-Simple Sequence Repeat (ISSR) Market
    (Bells University of Technology, 2017-10-26) NKANG, Nkoyo Ani; Dr. O. B. Ojuederie
    This study determined the genetic diversity in 20 Winged bean accessions. Five Inter Simple Sequence Repeat (ISSR) primers were screened and used to determine the genetic relatedness of the accessions of winged bean and also to identify the most polymorphic ISSR markers for future phylogenetic studies in Winged bean. Phylogenetic relations were determined by cluster analysis using unweighted pair-group method with arithmetic averages (UPGMA). Multivariate grouping was carried out using factorial coordinate analysis (FCoA) and polymorphic information content (PIC) was calculated using standard procedures. Four out of the five primers had consistent, well banded, reproducible profiles. The four ISSR primers; UBC 810, UBC 811, UBC 827 and UBC 855 generated a total of 127 amplified bands and all the bands were polymorphic (100%). The number of polymorphic bands per primer ranged from 14 (UBC 811) to 53(UBC 855). The primers identified between 9 (UBC 811) and 18 (UBC 855) of the 20 winged bean accessions. ISSR primer UBC 855 had the highest number of polymorphic bands (53) and identified the highest number of accessions (18). The UPGMA cluster analysis grouped the 20 accessions of the Winged bean into four major clusters and the dissimilarity distance ranged from 0.515 to 0.929. The FCoA analysis was comparable to that obtained using UPGMA cluster analysis. The PIC values ranged from 0.500 (UBC 811) to 0.874 (UBC 855) with an average of 0.718. The percentage polymorphism of 100% indicates high genetic diversity across the genomic loci of the accessions and revealed the usefulness of ISSR markers in determining the extent of genetic variability in Psophocarpus tetragonolobus.
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    Molecular Characteristics of Sesame (Sesamum indicum L.)
    (Bells University of Technology, 2017-10-26) KAYODE, Ayodeji Emmanuel; Dr. O. B. Ojuederie
    Forty-five (45) Sesame genotypes were collected from five farmers in three (3) major sesame producing states in Nigeria, which are positions in the North Central (Nasarawa), East (Bauchi), and Northwest (Kano states) of the six (6) geo-political zones of Nigeria. This was carried out using simple random sampling technique (SRS). Five simple sequence repeat markers (SSR) were screened and used to determine the genetic relatedness of the sesame genotypes. Phylogenetic relations were determined by cluster analysis using unweighted pair-group method with arithmetic averages (UPGMA). Multivariate grouping was carried out using factorial coordinate analysis (FCoA) A total of 99 n=bands were identified of which all were polymorphic. The number of amplified fragments per primers ranging from 25(SEMI10) to 27 (SM8). Polymorphic fragments were generated by SSR makers SM8, SEM10, and SM9, while primer SME18 and SME44 did not identify any genotype. The dendrogram grouped the genotype into six clusters with an overall similarity coefficient of 67%.Genotype Var01(KBil) collected from Bichi LGA of Kano state was genetically distinct from all other genotype at dissimilarity coefficient of 0.33. The FCoA placed the 45 genotypes of sesame into 6 groups with total of 25 genotypes. From the FCoA ,genotype var 01 (KBi 1) from Bichi LGA of Kano state, var 45 (NE 2) and var 32 (NL 2) from different farmers indifferent LGA of Nasarawa state, were the most distinct from the other genotype. The FCoA failed to differentiate genotype values to their area of origin. The PLC values ranged from 0.137(SEM9) -0.712 (SM8) and loci having PIC values closer to 1 are more desirable. Although 100% polymorphism was obtained for primers SM9, and SEM10, primer SM8 was most informative with the highest 0.712).