Theses/Dissertations/Projects

Permanent URI for this collection

https://ir.bellsuniversity.edu.ng/handle/123456789/230

Browse

Browsing Theses/Dissertations/Projects by Author "Dr. K. E. Ogunsola"

Now showing 1 - 3 of 3
  • No Thumbnail Available
    ItemMetadata only
    In Vitro Propagation of Olive Plant (olea europea) "SOURT" Using Nodal Culture
    (Bells University of Technology, 2016-08-19) KALEJAIYE, Olusegun Olanrewaju; Dr. K. E. Ogunsola
    This study was undertaken to contribute to the previous knowledge of the efficient in vitro protocols for the micropropagation of olive plant. This work was based on development of a new in vitro propagation method to overcome growth recalcitrance an olive cultivar "souri". An experiment to determine optimum sterilization procedures for olive explants was conducted. The culture medium used was composed of Murashige and Skoog (MS) medium without hormonal modification and MS medium modified with various levels of auxin-cytokinin concentrations. The experiments were laid out in completely randomized design (CRD) with 10 replicates per media treatment. Data were collected at 2,4 and 6 weeks of culture on growth parameters which include frequency of buds formed, frequency of callus formation, number of leaves, leave length, leaf width, number of roots and root length. Data were analyzed by ANOVA using SAS programme (9.2 version) and means were separated by the Duncan multiple range test at 5% probability. Of the different sterilization treatments used on the explants during the establishment of culture, a treatment of 70% ethanol for 3minutes and 5% Sodium hypochlorite (NaOCI) for 2minutes respectively proved to be the best sterilant combination for the Olive explants. This treatment resulted in 91% survival of explants and zero death of explant after 21 days of culture. The Olive plant had the best initiation and proliferation in culture medium containing 2.0 mg/l BAP (treatment 3) followed by the medium modified with 0.05 mg/l BAP + 0.02 NAA. The parameters considered as evidence of success was bud formation, callus formation, number of leaves, length of leaves and width of leaves. The mean number of shoots produced in the medium supplemented with 2.0 mg/l BAP was (2.3 # 0.2) while the mean number of shoots obtained from Olive nodal cutting in MS medium containing the hormonal modification with the second best effect was (1.6 # 0.1). This hormonal treatment with the best mean number of shoot production (2.0 BAP mg/l), had the highest mean shoot length of (6.5 ‡ 0.3 ) and a mean leave width of (3.1 # 0.2 ). Murashige and Skoog medium supplemented with 0.05 mg/l IBA + 0.02mg/l NAA (treatment 4), gave the Olive plant the best rooting effects. The highest root number was 3.8 # 0.9 and that of the root length was 2.5 # 0.7. This study provides a media protocol for the in vitro regeneration of the Souri Cultivar of Olive which will foster the application of tissue culture techniques in the micropropagation of Olive plant and also enhance improvement of the plant through transformation.
  • No Thumbnail Available
    ItemMetadata only
    Inheritance Pattern of Bean Common Mosaic Virus(BCMV) Resistance Gene Using Marker Assisted Selection and of Seed Coat Colour in Cowpea
    (Bells University of Technology, 2023-05-21) OSAKWE, Obiora Joseph; Dr. K. E. Ogunsola
    Cowpea (Vigna unguiculata (L.) Walp) is one of the most important grain legume crops in sub-Saharan African. Blackeye cowpea mosaic strain of the bean common mosaic virus (BCMV-BICM) causes mosaic infections that reduces the yield of cowpea Planting resistant varieties is the most effective control method but complex nature of disease resistance makes identification of resistant and susceptible plants cumbersome through conventional screening techniques .The use of marker Assisted selection (MAS) is more effective for crops genetic improvement. Seed coat colour is also an important trait in cowpea preference by consumers. Knowledge of the mode inheritance of viral resistance and seed colour are crucial in developing improved cowpea varieties. Inheritance of seed coat color and resistance to BCMV-BICM using MAS, was investigated using two improved cowpea breeding lines. Cowpea resistant (IT98K-1092-1) and susceptible (Ife-brown) varieties obtained from the international institution of Tropical agricultural (IITA), Ibadan were used. Crosses were made between the BCMV resistant (back seed) and susceptible (brown seed color) varieties. The F1 seed were advanced to F2. Genomic DNA of parent lines and F1 were extracted. MAS was used to determine the pattern of inheritance of virus resistance and chi square test of the colour segregation of F2 generations was used in investigating the inheritance of seed color. Data on morphology traits were also taken. There were no significant differences (p=0.01) between the two parental lines and between F1 and F2generations in the number of seed\pods, pod length (cm), seed weight, seed\plant, pod no\plant, seed number\plant, and germination percentage.
  • No Thumbnail Available
    ItemMetadata only
    Morphological Ploidy Analyses of Scent Leaf Plant (ocimum species) from South-South States in Nigeria
    (Bells University of Technology, 2019-04-30) FRANCIS, Bisi Eyitayo; Dr. K. E. Ogunsola
    Ocimum basilicum, commonly known as sweet basil or sent leaf plant, is an important aromatic plant of the Lamiacee family, most of which are medicinal. Among more than 150 species of Ocimum, sweet basil is the major essential oil crop commercially cultivated in many countries. It is commonly used to flavour foods such as vegetables, poultry and fish among other uses. However, there is limited information about the diversity and ploidy status of the plant which are required for plant improvement. This study was aimed at investigating the morphological diversity of scent leaf plant from six South-south States of Nigeria, identifying a standard for ploidy analysis of scent leaf plant through chromosome counting of a related specie and performing cytological analysis of Ocimum species for chromosome number and ploidy level. Seeds of scent leaf genotypes were collected from six states in the southern part of Nigeria. Twenty three samples were obtained in all. The seeds were raised in the nursery and later transplanted into planting bags containing 5 Kg sandy loam soil. Morphological data were taken on plant height (cm), leaf length (cm), leaf breadth (cm), leaf area, date of flowering, stem diameter (mm) and leaf shape from 12-to 20ª weeks after planting. The growth parameter Data were analyzed by ANOVA using Statistical analyses software (SAS) 2008 version. A related species of scent leaf plants was successfully used to prepare a standard for chromosome counting of the 23 accessions. This standard was eventually used to determine the ploidy levels of the different accessions form the six states and Ploidy analysis was carried out using Flow cytometer. Analysis showed high degree of variation among studied accessions indicating rich diversity represented within the populations from different phyto-geographical regions and relatedness among the species. The leaf margin for all the samples were serrated. Dates of flowering were within the range of 12 to 18 weeks.